Patients with recurrent infections, those with structural abnormalities of the urinary tract, those who have had urethral instrumentation, and those whose infections were acquired in the hospital have an increased frequency of infection caused by Proteus and other organisms e. Clinical expression[ edit ] Enterobacteriaceae of which Proteus is a member and Pseudomonas species are the micro-organisms most commonly responsible for Gram-negative bacteremia and sepsis.
Microbiology Unknown 1 was found in a nearby pond that was created by an earthquake. Some of the various methods introduced and practiced in class were applied in identifying the microbe.
Procedures were followed as stated in the index of the Virtual Unknown Software. First the shape and color of the microbe was noted.
This is done to know the morphology of the microbe. After concluding that the microbe was Gram negative rods, it was inoculated into a previously prepared test tube.
Table 1 shows the tests, purpose, reagents and results of the various tests performed. Each test was performed according to the index of the Virtual Unknown software and the following tests were performed: None Turned pink Negative adonitol test Cellobiose Fermentation To determine if the microbe can ferment the carbohydrate sugar cellobiose as a carbon source.
None Turned Yellow Positive cellobiose fermenter Maltose Fermentation To determine if the microbe can ferment the carbohydrate sugar maltose as a carbon source. None Turned Yellow Positive maltose fermenter Lactose Fermentation To determine if the microbe can ferment the carbohydrate sugar lactose as a carbon source.
None Turned Pink Negative lactose fermenter Raffinose Fermentation To determine whether the microbe can use sugar raffinose for carbon and energy. None Turned pink Negative raffinose fermenter Sorbitol Fermentation To determine if the microbe can ferment the carbohydrate sorbitol as a carbon source.
None Turned yellow Positive sorbitol fermentation Sucrose Fermentation To determine if the microbe can ferment the carbohydrate sucrose as a carbon source None Turned Pink Negative sucrose fermenter Melibiose Fermentation To determine if the microbe can ferment the carbohydrate sugar melibiose as a carbon.
None Turned Pink Negative melibiose fermenter Indole Production To determine whether the microbe can produce indole from the amino acid tryptophan. The reagent does not mix with water and forms a thin layer above the broth. The reagent reacts with idole to produce a cherry red ring.
Once the morphology of the microbe was identified, it was isolated and inoculated into a test tube. The nine tests performed on the microbe were done according to the index of the virtual unknown software and the results of each test correlated to the results anticipated.
As a result, it was concluded that the unknown 1 microbe was Citrobacter amalonaticus. Citrobacter amalonaticus has a gram negative rod-shape. They can be found in the digestive system and are linked with digestive disorders such as diarrhea.
They can also be present in the urinary tract and most commonly causes Urinary Tract Infections. Citrobacter amalonaticus is also known as an opportunist pathogen. Opportunist pathogens are pathogens that do not cause much damage to healthy individuals; however, it can cause very severe damage to those with a weakened immune system.
Besides being found in the intestines of humans, Citrobacter amalonaticus is usually found in the ground soilin the air and in the intestines of animals such as bats. It has the ability to recycle hydrogen.
In addition it is capable of breaking down nitrates into nitrites in the nitrogen cycle. This process is commonly used in the biodegration industry. There tannic acid degrades into tanneries. This allows for the harmful substances such as uranium to be removed from water, soil and uranyl phosphate crystals.
Citrobacter amalonaticus A cousin to Citrobacter Freundii.major unknown report san diego miramar college, how to write an unknown lab report in microbiology, proteus vulgaris unknown lab report brastrapsupporter, free proteus vulgaris unknown lab report pdf babyoid, identification of an unknown bacterium and writing up a report, microbiology 20 biochemical unknown guidelines spring , microbiology.
Microbiology Unknown Lab Report - Download as PDF File .pdf), Text File .txt) or read online. Identification of Proteus Vulgaris From an Unknown Sample.
Escherichia coli Klebsiella oxytoca Klebsiella pneumoniae Pseudomonas aeruginosa Pseudomonas aureofaciens Negative Proteus vulgaris Proteus mirabilis Serratia marcescens Morganella. Microbiology 20 Biochemical Unknown – Spring (due May 14th) You should be prepared to turn in your notebook with your biochemical unknown identification completed after lab on Thursday May regardbouddhiste.com of a possible 70 points you.
UNKNOWN LAB REPORT, MICROBIOLOGY. Unknown # and Alternate #9 Josephine Vitale May 5 th, Microbiology Lab.
Introduction. Correctly identifying microorganisms is an important and imperative process. Microbiology 20 Biochemical Unknown Guidelines – Fall You should turn in your biochemical unknown report by Tuesday November 24th. Out of a possible 70 points you will be graded as follows: Proteus mirabilis Proteus vulgaris Providencia alcalifaciens Pseudomonas aeruginosa.
This led us to believe that our unknown may be Escherichia coli, Enterobacter aerogenes, Proteus mirabilis, or Proteus vulgaris. See figure 2. 3/22/05 To differentiate between Escherichia coli, Enterobacter aerogenes, Proteus mirabilis, and Proteus vulgaris we decided to test carbohydrate fermentation pathways of the unknown sample.